WebIn order to assess the specific effects of VDAC1 during erythroid differentiation, we pursued a shRNA-mediated knockdown approach. CD34 + cells were transduced at day 4 of differentiation with a shSCR or a shVDAC1 lentiviral vector, both harboring the EGFP transgene (Online Supplementary Figure S1A), and EGFP + cells were sorted at day 7 . WebPlasmid PLKO-mcherry-luc-puro-shSCR from Dr. Carl Novina's lab contains the insert shSCR and is published in Mol Cell. 2010 Dec 10. 40(5):841-9. This plasmid is available through …
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WebFeb 27, 2024 · his table lists the standard operating hours and the exceptions to those standard hours throughout the year for the JMU's Student Success Center. WebNov 17, 2024 · Western blot assay showed that treatment of shScr MiaPaCa2 cells with a lethal dose of β-lap alone (6 μM) resulted in p53 proteolytic cleavage, diagnostic of programmed necrosis (lane 2, Fig. 5C), while cleavage of caspase 3/7 and cleaved PARP1 (89 kDa), diagnostic of apoptosis, were found in shXRCC1 cells exposed to 3 μM β-lap … opening amazon seller account
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WebMay 15, 2024 · The control of promoter-proximal pausing and the release of RNA polymerase II (RNA Pol II) is a widely used mechanism for regulating gene expression in metazoans, especially for genes that respond to environmental and developmental cues. Here, we identify Pol II associated Factor 1 (PAF1) as a major regulator of promoter … WebPort of Tanjung Perak is the second busiest sea port in Indonesia, located at Surabaya, East Java. It is the main port for the eastern part of the island of Java. The port is accessed … WebWhat does SHSC abbreviation stand for? List of 26 best SHSC meaning forms based on popularity. Most common SHSC abbreviation full forms updated in December 2024 iowa trout streams map