WebTo complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST cDNA Synthesis Kit. Efficient – high-target affinity, coupled with a novel … WebMar 1, 2024 · Total RNA was isolated using RNeasy RNA isolation kit (Qiagen) and reverse-transcribed by SensiFAST cDNA Synthesis Kit (Meridian Bioscience). RT-PCR and real-time RT-PCR were performed using Solg Taq DNA polymerase (SolGent) and PowerUp SYBR Green Master Mix (Applied Biosystems), respectively. Beta-actin was used as an internal …

SensiFAST cDNA Synthesis Kit 50 rxns – GC biotech webshop

Web• Size of cDNA —M-MLV can be used to synthesize first-strand cDNA up to 7 kb • Applications —synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR Source Purified from E. … WebMar 2, 2024 · Total RNA was extracted from the seeded macrophage cells (RNeasy Mini Kit, QIAGEN, Germantown, MD, USA) and reverse-transcribed into cDNA using the SensiFAST™ cDNA Synthesis kit (Bioline, Aust Pty. Ltd., Auburn, NSW, Australia). mao zedong personality type

SensiFAST cDNA Synthesis Kit - GC Biotech

WebThe complementary DNA (cDNA) was synthesized using SensiFAST™ cDNA Synthesis Kit (Bioline, Nottington, UK) according to the manufacturer’s protocol. The reverse transcription polymerase chain reaction (RT-PCR) was carried out using Solg™ Taq DNA polymerase (SolGent, Daejeon, Korea). The PCR conditions were as follows: initial denaturation ... WebSensiFAST™ HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on their DNA strand dissociation behavior, with increasing temperature, in the … WebThe SuperScript™ Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. krack cooling units