Webconsists of the incorporation of the photocleavable fluorescent nucleotide into the DNA, detection of the fluorescent signal, and photocleavage of the fluorophore. The entire process was re-peated to identify 12 continuous bases in the DNA template. These results demonstrate that photocleavable fluorescent nucleotide WebMeaning of photocleavable. What does photocleavable mean? Information and translations of photocleavable in the most comprehensive dictionary definitions resource on the web.
Photolabile protecting group - Wikipedia
WebJan 6, 2003 · The 2-nitrobenzyl group is widely used in organic synthesis as a protecting group and a cleavable linker because of its high photocleavage efficiency by near-UV light irradiation (1–4).A cleavage quantum yield of 0.49–0.63 has been reported in the literature for 1-(2-nitrophenyl)ethyl phosphate esters ().This unique property of the photocleavable … WebMay 31, 2024 · Photocleavable molecules can enable the light-dependent modulation of biomolecular activities with high spatiotemporal precision. We have previously reported a … cup to liter ratio
Photocleavable linkers: design and applications in …
WebAbstract. To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase ... WebAug 1, 1995 · Abstract. While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm ... Webunique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphora-midite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry (8, 9). cup toon 2020