Biotin switch assay
WebDec 2, 2016 · Using arsenite as reducing agent in the modified biotin switch assay, we were able to quantify 1161 proteins, in which more than 100 sulfenic acid sites were identified. Bioinformatics analysis of the quantified sulfenic acid sites highlighted the relevant, previously missed biological process of monocyte transendothelial migration, … WebS-nitrosylation (or S-nitrosation, SNO) is an oxidative posttranslational modification to the thiol group of a cysteine amino acid residue. There are several methods to detect SNO modifications, mostly based on the classic biotin-switch assay, where the labile SNO sites are replaced with a stable biotin moiety to facilitate enrichment of the modified proteins.
Biotin switch assay
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WebJun 12, 2001 · Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. Here, we describe a novel method for detecting proteins that contain nitrosothiols. In this three-step procedure, nitrosylated cysteines are converted to bio … WebClassic biotin-switch assay and its derivatives are the most common methods used for detecting SNO. In this approach, the labile SNO group is selectively replaced with a …
WebThe biotin-switch assay was then performed as described above. Quantification and statistical analysis. Fiji (ImageJ) and Image Studio were used for quantification of immunohistochemistry and immunoblot data, respectively. A Power Analysis of our prior data was used to determine the number of replicates needed for statistical purposes. WebAbout This Assay Cayman’s S-Nitrosylated Protein Detection Assay Kit (Biotin Switch) employs a modification of the Jaffrey et al. “Biotin-switch” method to allow for the direct …
WebJul 3, 2024 · Biotin Switch Assay Kit (S-Nitrosylation) (ab236207) employs a modification of the Jaffrey et al. 'Biotin-switch' method to allow for the direct visualization of S-NO …
WebMar 1, 2004 · Biotin switch assay, Western blot, and protein purification The biotin switch assay was performed essentially as previously described [22] , [42] . Extracts were adjusted to 0.5 mg/ml of protein and equal amounts were blocked with 4 volumes of blocking buffer (225 mM Hepes, pH 7.7, 0.9 mM EDTA, 0.09 mM neocuproine, 2.5% SDS, and 20 mM …
WebMar 3, 2024 · The biotin-switch assay was performed as previously described, using BME as the reductant (Wang et al., 2014). The time-course assay was carried out as described in Wang and Sevier (2016). In brief, CSY622 or CSY625 were grown to mid-log phase in SMM at 30°C and were treated with 5 mM diamide for 15 min. Diamide-containing medium … northern virginia legal directoryWebDownload scientific diagram Modified biotin switch assay. from publication: Immunoprecipitation methods to identify S-glutathionylation in target proteins S-glutathionylation is a reversible ... northern virginia magazine best restaurantsWebFeb 21, 2012 · The biotin switch assay followed by Western blot or immunoprecipitation using an S-nitrosocysteine antibody was also employed to validate S-nitrosylated proteins in endothelial cell lysates. Results Seventy-eight potential S-nitrosylated proteins and their target cysteine residues for S-nitrosylation were identified; 9 of them were Golgi ... northern virginia law firmsWebMar 14, 2024 · The biotin-switch assay showed that BR treatment clearly induced the oxidation of BZR1, and application of H 2 O 2 enhanced the oxidative effect ... northern virginia ldiWebDec 10, 2016 · The biotin switch assay has been widely applied to the quantitative analysis of S-nitrosylation in different disease models and is now also emerging as a valuable research tool for other oxidative ... northernvirginiamagazineWebMay 19, 2024 · Biotin switch assay for detection of S-nitrosylation. S-nitrosylated proteins were detected using biotin switch assay as reported previously [25,26,27]. In brief, cells were incubated with ... northern virginia legal aid societyWebJun 12, 2001 · Purification of biotinylated proteins. 1. To the samples from "Biotinylation of nitrosothiols," add two volumes of -20°C acetone and incubate for 20 min at -20°C to … northern virginia magazine event listing